ENZYME WEBQUEST
Choose ENZYMES: The BASICS
1. SLIDE ONE: Enzymes are ________________that serve as __________________.
They _________________up or _______________ ______________reactions, but ALWAYS remain _________________.
2. SLIDE TWO: What is an ACTIVE SITE?
3. SLIDE THREE: Each ______________acts on a specific_____________.
4. What is a SUBSTRATE?
5. DRAW an ENZYME and SUBSTRATE below. Make each a DIFFERENT COLOR and label each.
6. SLIDE FIVE: YOU JUST DRANK A GLASS OF MILK! DRAW THE CATALYTIC CYCLE OF LACTOSE BELOW! LABELING THE SUBSTRATE, SUBSTRATE ENZYME COMPLEX, THE ACTIVE SITE, AND THE PRODUCTS. MAKING EACH A DIFFERENT COLOR!
7. Someone who is “LACTOSE INTOLERANT”…is lacking which enzyme?____________________
Enzyme Catalysis
Key Concepts
8. _________________ catalyze reactions by lowering the _______________ _________________ necessary for a reaction to occur. The molecule that an enzyme acts on is called the _________________. In an enzyme-mediated reaction, _________________ molecules are changed, and _________________ is formed. The _________________molecule is _________________ after the reaction, and it can continue___________________________________________________________________________________________________________________________________.
9. Each _________________ is _________________for the _________________ it will catalyze. In this laboratory,
Substrate = hydrogen peroxide (H2O2)
Products = water and oxygen
10. DRAW THE REACTION THAT TOOK PLACE ON THE LEFT USING YOUR OWN SHAPES, LABELING THE SUBSTRATE, ENZYME, ACTIVE SITE ON THE ENZYME, ENZYME-SUBSTRATE COMPLEX, AND THE PRODUCTS.
Enzyme Structure
11. Enzymes are globular, functional _____________________. Their folded conformation creates an area known as the _________________ _______________. The nature and arrangement of __________________ ____________________ in the __________________ __________________ make it specific__________________________________________________________.
12. DRAW A MODEL OF AN ENZYME, ACTIVE SITE, AND SUBSTRATE BELOW:
Some Factors That Affect Enzyme Action
13. The conformation of an enzyme is maintained by interactions between the various ___________________ _______________ that compose it, and this conformation is sensitive to ________________________________________. Two important influences are _________________ and ________________. When an enzyme's ________________________ is significantly altered because of ____________ or ___________________ variation, the enzyme may no longer _____________________ ___________________. An enzyme is said to be _____________________ when it loses its functional shape.
pH and Enzyme Function
14. Each enzyme functions best within a _______________________. For example, the enzyme__________________, which works in your stomach, functions best in a strongly ____________________environment. Lipase, an enzyme found in your _____________________________, works best in a __________________ environment.
15. When the pH changes, the active site ____________________________ and affects ____________________ ________________. What happens to catalysis when an enzyme is subjected to a pH far from its optimum range?
16. WATCH THE ANIMATION AND DESCRIBE WHAT YOU SEE BELOW:
Temperature and Enzyme Function
17. Chemical reactions speed up as ____________________ is increased, so, in general,_________________ will ___________________ at higher temperatures. However, each enzyme has a temperature __________________________, and beyond this point the enzyme's _____________________________ is lost. ___________________ temperatures will ______________________ most enzymes.
WEBSITE#3
GO TO ANIMATION, then to STEP THROUGH
1st-Add a SUBSTRATE
2nd-Add an ENZYME
PRESS PLAY
18. Describe what happened:
Make sure you are in the ANIMATION portion and go to STEP THROUGH…Watch the animation…it is a bit complicated. Main idea I need you to understand is….
19. TRUE or FALSE: an enzyme can change shape and become ACTIVE or INACTIVE.
